The rubber tree is a plant native to Amazon/Brazil, which has as main commercial interest the extraction of latex for the production of natural rubber. Thus, a large amount of by-products from this extraction is discarded every year. One of them are the seeds that have an oil, which could be applied in different industrial sectors such as pharmaceutical, cosmetic and food. Some studies show the presence of fatty acids in the oil of rubber trees all around the world, extracted using conventional techniques with organic solvents. However, it is scarce the knowledge of the composition of the oil of the seeds from Brazil. Due to this, the objective of this work was the extraction of rubber tree seed oil using Soxhlet and supercritical carbon dioxide, through an experimental design, varying temperature (40 to 80 ºC) and pressure (20 to 50 MPa). The best extraction yield was at 50 MPa and 60 ºC, showing that pressure has a significant influence over the oil yield and solubility. The supercritical extraction showed a high selectivity for the squalene extraction. At higher pressures, the -linolenic acid is favored to be extracted, although it was not identified in the Soxhlet extraction.

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