Antioxidant potential, anti-α glucosidase, anti α β-Glucuronidase and anti tyrosinase activity of Hippophae rhamnoides and Cassia fistula Extracts were evaluated. Antioxidant activities were tested using 2,2-diphenyl-1-picrylhydrazyl radical, ferric-reducing antioxidant power, Superoxide radical scavenging assay, Hydroxyl radical scavenging assay and Nitric oxide radical scavenging assay. ß-Glucuronidase activity was determined by measuring the absorbance at 405 nm of p-nitophenol formed from the substrate by spectrophotometric method of R. A. Collins. The α-glucosidase inhibition and Antityrosinase activity was determined using Synergy HT BioTek 96-well plate reader method. Both plants extracts exhibited appreciable total phenolic contents i.e. Hippophae rhamnoides 51.23±1.38 and Cassia fistula 64.39±1.21 mg gallic acid equivalents/g. Similarly both plants extracts showed appreciable antioxidant potential and statistically insignificant differences (P > 0.05) were observed among the triplicates in different systems of antioxidant activity evaluation. Marked ß-Glucuronidase, α-glucosidase and Antityrosinase activity was observed for both plants extracts. This study revealed that Hippophae rhamnoides and Cassia fistula could contribute significantly in the management and/or prevention of degenerative diseases linked to free radical damage as well as in diabetics, skin related disorders like melasma, in rheumatoid arthritis and in biliary cholelithiasis.

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