The present study aimed to investigate the different responses of sensitive and tolerant \nrice genotypes under water stress condition induced using poly ethylene glycol (PEG \n6000). The studied genotypes were Giza177, Giza181, Giza182 and Sakha103 \n(sensitive), Sakha104 (moderate), Orabi2 and N38 (tolerant). The present \ninvestigation included determination of germination %, fresh and dry weight, shoot \nand root length, cell membranes stability as rate of electrolyte leakage (EL), lipid \nperoxidation as MDA content. Semi-quantitative reverse transcriptase polymerase \nchain reaction (sqRT-PCR) for some protective proteins such as heat stable proteins \n(HSPs) and Dehydrins (DHNs) standardized on actin transcript amounts were carried \nout to investigate the changes in the expression pattern of those genes. All \ndeterminations were carried out for all studied genotypes under both control and \ndrought conditions. The obtained results revealed that water stress tolerance of \ntolerant genotypes was accompanied with decreasing of electrolyte leakage rate and \nlow content of MDA comparing with sensitive ones. sqRT-PCR analysis for \nexpression pattern of studied genes showed increasing in the expression of HSP-13, \nHSP-12, HSP-9 and DHN-2 in the seedling of tolerant genotypes comparing with \nsensitive ones. The present study pointed to the participation of those genes in the \nacquisition of drought tolerance in tolerant genotypes.

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