It was assumed that using of green synthesis approach is the best way for the synthesis of nanoparticles being recovered from utilization of toxic compounds and also preserving of natural capping agents for stabilization of nanoparticles. At this study, the effect of different concentrations of silver nanoparticles from Cynanchum acutum L. latex and its crude latex on molecular DNA level, protein profile and mitotic division of Vicia faba seeds was demonstrated. Results revealed that silver nanoparticles were formed and monitored at 432 nm. UV–visible spectroscopy and transmission electron microscope (TEM) were operated to characterize the synthesized silver nanoparticles (SNPs). The majority of the particles were spherical in shape. The highest value of mitotic index (MI %) was recorded for treated Vicia faba with 25 ppm silver nanoparticles. Cytotoxicity was observed via several types of chromosomal abnormalities such as micronucleus at interphase, disturbed at metaphase and anaphase, fragments, chromosome stickiness, bridges and laggards. SDS-PAGE banding pattern of proteins in treated seeds of V. faba consists of sixteen bands with ten monomorphic bands and six polymorphic bands resulted in total percentage of polymorphism 37.5%. On the molecular level, eight ISSR primers were used producing a total of 79 bands with 56 polymorphic bands and 23 common bands. The highest percentage of polymorphism (92.86 %) was recorded for primer ISSR14 having one monomorphic band and thirteen polymorphic bands divided into four unique and nine non unique bands. All treatments caused reduction in genomic template stability values; comparing with control and the increase in AgNPs concentrations showed decrease in GTS % values. These results supposed that AgNPs had significant toxic effects on V. faba plants at cytological, biochemical and molecular levels. The lowest value of GTS % using SDS-PAGE and ISSR were recorded for 100ppm silver nanoparticles treatment.

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