This article proposes to design a wafer with integrated vertical cross-shaped asymmetric electrodes and Y-shaped microfluidic channels. The micro-particles are separated and captured in the microfluidic pipeline. From the experiment, the front end is used to observe the separation of microparticles. Then, the back-end capture of the microparticles is carried out in order to achieve better detection applications. Among them, the inlet 1 of the pipeline is the NaCl solution containing particles of different sizes under the influence of the asymmetric electrode at the front end, and the inlet 2 of the other pipeline is only injected with the NaCl solution, and the two merge into the third pipeline and then flow to an expansion pipeline together. After the particles pass through the expansion duct, the smaller particles will be brought to the recirculation zone due to the influence of the asymmetric electrode pair, and will continue to orbit inside the recirculation zone with the reflow. With the integrated chip design of the front and back ends, micro-particles or cells can achieve the effect of double particle separation and capture after passing through this section.

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