Background: Hospitals have many Hazards, includes occupational hazards and iatrogenic hazards. Fungal pollution in laboratory is wide spread . Health care worker and students had high risk for acquiring infections during their clinical training and work . Therefore, this study aim is to develop safety rules protocol to protect students of Colleges of Applied Medical Science in Bisha University and health care workers from Airborne Fungal Infection. Sample: A convenience sample of 40 students from Colleges of Applied Medical Science and all healthcare workers. There are also 50 jury group. Design: Queasy experimental study. Tools: Three tools were used for data collection, namely; a validity form, interview questionnaire sheet, observation checklist and fungal spores isolation on sabouraud̕s dextrose chloramephenicol agar and sabouraud̕s dextrose cyclohexamide agar at 28⁰C. Results: The result revealed that the developed safety protocol is valid to be used in microbiological laboratory and the number of airborne fungi decreased after the implementation of safety rule protocol. Conclusion and recommendation: The developing safety rules protocol is valid to be used in microbiological laboratory and decrease number of air born fungi after intervention . It is recommended to disseminate of the developed protocol to prevent fungal infection in microbiology laboratories.

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