Human teeth enamel, the hardest tissue in the body, is produced at embryo development during amelogenesis. Its lack of regeneration after the teeth eruption and its destruction susceptibility turn it as a good subject of study. Ameloblastin an abundant, phosphorylated, proline/glutamine-rich protein, secreted during enamel formation is a structural matrix component that participates on the maintaining of prismatic structure of the enamel crystals. It also, has been involved in the animal amelogenesis as adhesion protein, expressed at pre-secretory stage that diminishes in the secretory stage. However its function and distribution in human being remains unclear. In order to contribute to the understanding of ameloblastin distribution during amelogenesis, the present study explores its location in the different structures on human fetal tooth by immunohistochemical labeling using a polyclonal antibody against ameloblastin in 5µm human jaws fetuses’ sections. Thus, at pre-secretory stage ameloblastin was detected at the apical membrane and also at basal end of the ameloblast, as well as in the stellate reticulum. At the secretory stage is located at the secretory ameloblast, enamel, and dentin and in the odontoblasts. This ameloblastin location can be related with the enamel crystals organization in both pre-secretory and secretory stages.

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