The effect of fluoride dentifrice (FD) and herbal non-fluoride dentifrice (HNFD) on the adherence of Streptococcus mutans to the surfaces of composite resin (CR) and resin sealant (RS) was evaluated. CR and RS specimens (n=36) (6x2mm), submitted to biofilm formation (1.5×107 CFU/mL) placed in Brain Heart Infusion (BHI) broth containing 1% sucrose. Samples were incubated at 37°C, 5% CO2 for 24 h or 7 days. After, specimens were removed from the medium and placed in 1mL of the dentifrice solution or sterile saline (Control) for 60 seconds. The remaining biofilm was collected, and the suspensions were serially diluted and plated on BHI agar to enumerate viable bacteria. Data were analyzed using three-way ANOVA and Tukey’s test (α=0.05). A significant difference in the time × treatment interaction was observed compared to the control (p<0.01), while no effect of the material was observed. A decrease in the number of cells adhered in both groups and evaluation times was observed compared to the control group (p<0.05) in the groups treated with both dentifrices in the 24h. The dentifrices assayed in this study equally affected the adherence of S. mutans to the surface of restorative materials.

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