Introduction: More than 60 million people in the United States have had an increase in their life expectancy due to the therapy of artificial tissues and organs, with every five people over 65 years old benefiting from in vitro generated tissues and organs. Only in 2016, the Unified Health System (SUS) spent more than 600 million reais with orthoses and prostheses. Objective: to respond to several information gaps by analyzing the biocompatibility of chitosan-collagen-genipine matrices in the presence of mesenchymal stem cells of human adipose tissue, investigating the intrinsic characteristics of these cells as the ability to differentiate the cultured cells in osteoblates, chondroblasts and adipocytes, immunophenotyping and proliferation in the biomatrixes. Methods: Ethics: The present study was evaluated and approved by the Research Ethics Committee of the Beneficência Portuguesa Hospital of São José do Rio Preto / SP, under number of approval process 030427/2015. Participants: For the development of the present study, two patients with ages between 20 and 30 years and candidates for an aesthetic procedure of liposuction were selected and accepted to donate the biological material (liposuction - adipose tissue) for the sole use of in vitro studies. Both patients signed the informed consent form. Results: The AMSCs maintained their characteristics of adhesion, proliferation, immunophenotyping and differentiation after culture in the biomatrixes, both before and after cryopreservation.Conclusion: The chitosan-collagen-genipin biomatrixes offer biocompatibility for adhesion, proliferation and biostimulation for the expression of cellular functions, mainly to stimulate the paracrine effects that lead to the process of tissue regeneration.

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