The objective of the present study was undertaken to evaluate the effect of pumpkin oil and olive oil and their combination on Gentamicin sulphate “GS”-induced nephrotoxicity in adult male albino rats(Sprague –Dawley strain). 40 adult male albino rats were used in this study (8 rats/group).Group one “control” (C) was injected with sterile saline; group two (GS) was injected with GS at a dose (100mg/kg), intra-peritoneal (i.p.) for 7 consecutive days, group three (GP) was injected with GS and supplemented with “pumpkin oil” (288mg/ 1Kg b.wt/daily) ,group four (GO) was injected with GS and supplemented with “Olive oil” ( 1.5 ml/ 1Kg b.wt/daily) and group five (GPO) was injected with GS and supplemented with “pumpkin oil” plus “olive oil” the experiment last for one month. Serum urea, creatinine and uric acid levels, oxidative stress parameters, and histopathological examination were investigated. Results revealed (1) significant decrease in TAC and SOD and significant increase in MDA , ALP ,urea, creatinine ,uric acid, and histopathological alterations in kidney tissue (light microscopy) in group exposed to GS. (2) Significant improvement in the measured parameters of groups subjected to GS and supplemented with pumpkin oil and olive oil. Conclusively, it was obvious that the supplementation with pumpkin or/and olive oil to rat exposed to oxidative stress was associated with improved, anti-oxidative parameters, kidney function, and histology of renal tissue.

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