Hippophae Antifungal Activity FTIR Spectroscopy Sea Buckthorn Natural Products

The present research study was designed to characterize the antifungal activity, minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and FTIR spectra analysis of various extracts of seabuckthorn leaves (SBL). Aqueous, methanol and ethanol extract zone of inhibition ranging from 09 – 16mm, acetone extract showed activity against two fungus F. oxysporum (12 ±1mm) and A. alternata (09 ± 0mm). While ethyl acetate, chloroform and n-hexane were found no zone of inhibition. The maximum and minimum MIC values for aqueous, methanol, ethanol and acetone extract were 80 – 180mg/ml. The MFC value for aqueous, methanol, ethanol and acetone extract were in ranging from 140 - 360mg/ml. F. oxysporum and A. paraciticus was the most susceptible fungus tested and P. digitatum was the most resistant fungus tested against SBL extract. The FTIR spectra of the SBL extract revealed the presence of different functional groups ranging from OH stretching for hydroxyl group, alkanes (C-H), alkenes(C=C), aromatic rings (C=O), carboxylic (C=O) and amides (aromatic).

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