gene expression RNA sequencing eukaryotic cells oxidative stress pathway enrichment regulatory networks

Gene expression regulation is fundamental to cellular response mechanisms under variable environmental stressors. This study aims to elucidate the dynamics of gene expression in eukaryotic cells when subjected to osmotic and oxidative stress. Utilizing high-throughput RNA sequencing, we analyzed the expression profiles of key regulatory genes across multiple time points. The methodology included differential expression analysis and pathway enrichment to identify significant changes. Our findings reveal a coordinated response involving upregulation of heat shock proteins and antioxidant enzymes, suggesting a conserved mechanism for stress adaptation. Furthermore, we identified novel candidate genes potentially involved in stress tolerance, broadening the understanding of cellular resilience. The study concludes by highlighting the complexity and interconnectivity of gene regulation networks, paving the way for future research in stress genomics and potential biotechnological applications.

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