Pseudomonas aeruginosa is the most common and ubiquitous pulmonary pathogen of humans, causing unscrupulous infections in patients with suppressed immune systems across all age groups. It is considered a key pathogen in numerous studies. This pathogen is responsible for significant mortality and morbidity globally due to several virulence factors and high rates of infections. Pseudomonas aeruginosa is notably lethal and persistent, causing acute ventilator-acquired pneumonia (VAP). The Quorum Sensing (QS) system in Pseudomonas aeruginosa regulates the expression of various virulence factors. This study aimed to detect the lasR gene associated with acute pulmonary infections in Pseudomonas aeruginosa. Samples, including endotracheal tract secretions and blood from patients with VAP, were provided by the Microbiology Lab of GCU Lahore. Five strains of P. aeruginosa were identified as highly virulent after confirmatory tests for antibiotic sensitivity and virulence assays. These pathogenic strains were used for ribotyping of the lasR gene. PCR products and sequence analysis using BLAST at NCBI confirmed the 100% presence of the lasR gene in the genome of P. aeruginosa strains involved in multiple virulence factors responsible for acute pulmonary infections. This study investigates the contribution of the lasR gene in pathogenic P. aeruginosa responsible for acute pulmonary infections.

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