This work evaluated the effect of Callicostella pallida extract (CP) incorporated into a microemulsified system on MCF-7 human breast cancer cells and co-culture of these cells with blood MN cells. The MCF-7 cells (ATCC) and the human blood mononuclear (MN) cells were used. MN cells, MCF-7 cells and co-culture (MN and MCF-7 cells) were pre-incubated for 24 h with or without CP or CP incorporated into the microemulsified system (CP-ME). The viability, superoxide, SOD, IL-4 and TNF-? in culture supernatant, and the apoptosis and necrosis were determined. There was a reduction in the SOD when the MCF-7 cells were incubated with CP, whereas there was increased SOD when the cells were treated with CP-ME. In co-culture, the SOD was higher when these cells were treated with PC-ME. The superoxide was higher in MN cells, MCF-7 cells and co-culture incubated with CP-ME. The TNF-? was higher in co-culture. The apoptosis and necrosis were higher in co-culture in cells incubated with CP-ME. These data suggest that CP-ME was able to activate cellular oxidative stress and induce the release of TNF-? and consequently cell death in co-culture of MN and MCF-7 cells, and CP-ME can be considered promising for future anti-tumor therapies.

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