Objectives: Amyotrophic lateral sclerosis is a chronic degenerative disease that affects motor neurons, thereby promoting functional changes in the human body. The study evaluated the electromyographic fatigue threshold of the masseter and temporalis muscles of subjects with amyotrophic lateral sclerosis. Methods: A total of eighteen subjects were divided into two groups: amyotrophic lateral sclerosis (n = 9) and disease-free control (n = 9). The groups were equally divided according to gender (7 male, 2 female). The fatigue threshold was analyzed using median frequencies obtained during the 5-second window (initial (IP), mid (MP), and final (FP) periods) of electromyographic signaling of the masseter and temporalis muscles bilaterally, with reduction in muscle force during maximal voluntary dental clenching. Results: A significant difference (p ≤ .05) in the left temporal muscle: IP (p = .05) and MP (p = .05) periods was demonstrated. The amyotrophic lateral sclerosis group showed a decrease in median frequency of the electromyographic signal of the masseter and temporalis muscles compared to the control group. Conclusion: Amyotrophic lateral sclerosis promotes functional impairment of the stomatognathic system, especially at the electromyographic fatigue threshold of the masticatory muscles.\nKeywords. Amyotrophic lateral sclerosis, electromyographic fatigue, median frequency, masseter muscle, temporal muscle

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