The study evaluated the effects of bioactive compounds of Anacardium humile ethanolic extract (AHEE) adsorbed on polyethylene glycol (PEG) microspheres on the oxidative stress of mononuclear blood cells (MN) in co-culture with MCF-7 breast cancer cells. MCF-7 cells, blood MN cells and the co-culture were pre-incubated with AHEE, PEG microspheres or AHEE adsorbed on PEG microspheres (PEG-AHEE). The antioxidant capacity of the AHEE compounds, bioassays were determined. AHEE contained DPPH (22 ?mol trolox.g-1), ABTS (45.2 ?mol trolox.g-1), FRAP (163.4 �mol trolox.g-1), carotenoids (69 �g ?-carotene.g-1 and, total phenolic compounds (189.8 mg galic acid.100g-1). 11.47% of total identified phenolic compounds were flavonoids, of which 11.17% was expressed as flavanol epicatechin and 0.30% as flavonol quercetin. Phenolic acids, expressed as chlorogenic acid, represented 0.91% of total phenolic compounds. The viability and rheological parameters were similar between cells and their co-culture. Superoxide decreased in MN and MCF-7 cells treated with PEG-AHEE microsphere but increased in the co-culture. The results suggest that Anacardium humile extract adsorbed on PEG microspheres displays antioxidant capacity and can modulate the oxidative stress of blood MN cells in co-culture with MCF-7 cells.

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