📚 Volume 26, Issue 5
📋 ID: b2mVfRB
Authors
Chien-Han Hsu 1 and Kai-Chieh Chen 2
1 Division of Internal Neurology, Chang Bing Show-Chwan Memorial Hospital, Changhua, Taiwan; 2 School of Medicine, Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan.
Abstract
Corchorus olitorius L. (COL) has abundant polyphenolics, which confer significant antioxidant activity. However, the detailed regulatory mechanisms remain unclear. To elucidate the bioactivities of COL, this study evaluated the effects of COL on TiO2-induced nephrotoxicity. The effect of COL on Kidney (MDCK) cells that had been damaged by TiO2 was evaluated using an MTT assay. MDCK cells were pretreated with rising concentrations of TiO2 (10-60 g/mL) to determine an appropriate concentration for subsequent treatment with COL. In this study, 40 g/mL of TiO2 was chosen as morphological changes were found in cells treated with TiO2 after 30 min. After treating with TiO2, EAS was subsequently added to the wells and the cells were reincubated for 24 h. We found that 0.1 and 0.2 mg/mL COL attenuated the effect of TiO2-induced cellular damage in MDCK cells. Taken together, our results revealed that COL protects MDCK cells against TiO2-induced cell damage by preventing ROS-dependent cell death.
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📝 How to Cite
Chien-Han Hsu 1 and Kai-Chieh Chen 2 (2019).
"Corchorus olitorius L. extract ameliorates TiO2-induced oxidative stress in kidney cells".
Wulfenia, 26(5).
